J. Phys. Soc. Jpn. 76 (2007) 024009 (6 pages)  |Previous Article| |Next Article|  |Table of Contents|
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Infrared Spectroscopy on Poly(dG)–Poly(dC) DNA at Low Hydration

Hasanudin Abdurakhman^1,2,3,4, Kenji Tajiri³, Hiroyuki Yokoi³, Noritaka Kuroda³, Hiroshi Matsui^1,2, Takeshi Yanagimachi², Masateru Taniguchi⁵, Tomoji Kawai⁵, and Naoki Toyota^1,2

(Received August 10, 2006; Accepted December 15, 2006; Published February 13, 2007)

Infrared absorption measurements have been made on dry samples of poly(dG)–poly(dC) DNA at various relative humidity and temperatures. The water content, controlled by the relative humidity, reduces as temperature increases and reaches a very low value of ∼0.1 wpn above 120 °C. This minimum water content is maintained when the samples are brought back to room temperature as long as they are kept at the relative humidity of ∼0%. The molecular vibrations, which characterize the backbone structure as well as the base stacking and pairing, indicate that our samples maintain an A-form double helical structure at all the values of water content. The disorder in the base stacking is observed as the result of the decrease of the water content. Additionally the denaturation appears at high temperatures above 100 °C, which reversibly disappears with decreasing temperature. ©2007 The Physical Society of Japan

KEYWORDS: DNA, structure, infrared, water content, molecular vibrations
URL: http://jpsj.ipap.jp/link?JPSJ/76/024009/
DOI: 10.1143/JPSJ.76.024009

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